Even though you’re well aware of peptides, have you heard about the “synthetic peptides“? Well, they are made by experts at the laboratories. Besides that, these peptides are not made for human use.
It’s mainly because they are used for research-related work and also play the part of epitopes to prepare vaccines. Since they have poor immunogenicity and small size, these peptides are attached to big carriers.
These carriers are known as liposomes, proteins, and synthetic polymers. The process of attaching carriers, such as liposomes, to peptides was established back in 1982 by “Papahadjopoulos and Martin.”
The Uses of Synthetic Peptides
There are several uses for synthetic peptides, and some of these are:
Generate all the Custom Antibodies
Experts can easily utilize the form of antigens to create custom antibodies. When it’s attached to the carrier, such as protein, it will stimulate the host’s humoral-resistant response.
Besides that, it will also help in generating both polyclonal and monoclonal antibodies. This type of method will enable you to have full control over the epitope easily when compared to utilizing the entire native protein.
The sequence of the amino acid of the antigen is extremely vital. Even though it’s an inexact science, you will certainly find tools through which you can choose the sequence to produce an immune response.
Used for Conducting Protein-to-Protein Communications
Synthetic peptides are ideal for breaking down all the basic components of protein-to-protein contact. The “Biotinylated Peptides” can be easily halted on the streptavidin-coupled drops.
After that, experts can then utilize it to pull down all those proteins that intermingle with the peptides. It’s mainly used in epigenetic analysis.
In this research, all the peptides that blend to histone tails without or with the post-translational changes get incubated with the nuclear extracts. All the associated proteins get analyzed through the “SDS-PAGE.”
Besides that, these types of peptides are also great for processes such as “Fluorescence Anisotropy.”
Here, having a smaller size is the main asset. Another usage of these peptides is when they are used as substrates for enzymatic responses. Experts have pointed out that back in 2019, Sugiyama et al. utilized the purified kinases and the peptide libraries to detect the kinase substrates.
This might not have been possible through the cellular systems and when looking for the relations of the recombinant or native proteins.
The Mass Spectrometry
Synthetic peptides are also used as the standards for mass spectrometry. It helps in the process of identification and quantitation.
When the protein gets analyzed and digested through the mass spec, it becomes difficult to quantify the peptides.
It’s primarily due to the mass spectrometry peak that depends heavily on the chemical makeup and concentration of the peptides.
Wrapping Up
Synthetic peptides are especially created to create various applications in the area of bioengineering and biotechnology fields. It’s also created only for research purposes and not designed for human usage.
If you’re a researcher or a scientist, you should buy peptides from a well-known and reliable shop online. Doing so will help you get the best quality product for your research work.
